Journal: Assay and Drug Development Technologies

Article Title: High Content Screening Characterization of Head and Neck Squamous Cell Carcinoma Multicellular Tumor Spheroid Cultures Generated in 384-Well Ultra-Low Attachment Plates to Screen for Better Cancer Drug Leads

doi: 10.1089/adt.2018.896

Figure Lengend Snippet: Characterization of HNSCC MCTS morphology and viability in Ultra-low Attachment Plates. Eleven HNSCC cell lines and Het-1A cells were seeded at 2,500 cells per well in 384-well ULA-plates and cultured for 3 days. MCTSs were then stained with the live cell CAM and dead cell EHD reagents, and 4 × images were acquired on the IXM in the TL, FITC, and Texas Red channels. Grayscale TL images are presented along with color composite fluorescent images of live cell CAM and dead cell EHD staining depicted as green and red, respectively. Representative images from multiple independent experiments are presented. All scale bars represent 300 μm. CAM, Calcein AM; EHD, Ethidium Homodimer; HNSCC, head and neck squamous cell carcinoma; IXM, ImageXpress Micro; MCTS, multicellular tumor spheroid; TL, transmitted light; ULA-plates, ultra-low attachment microtiter plates.

Article Snippet: Generation of HNSCC MCTSs in Ultra-Low Attachment Microtiter Plates We have previously described the generation of MCTSs after seeding several HNSCC cell lines into 384-well U-bottomed ultra-low attachment microtiter plates (ULA-plates; Cat. No. 4516; Corning, Tewksbury, MA).

Techniques: Cell Culture, Staining